Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 Pancreatic stellate cells as a morphological basis for the development of pancreatic fibrosis Панкреатичні зірчасті клітини як морфологічна основа розвитку фіброзу підшлункової залози 5 12 UK O. Yu. Sirenko 2010 1 20 2010 3 6 Last decade in Ukraine and many countries there is a clear tendency to increase the number of cases of pancreatic diseases. For more than century study of the pathogenesis of chronic pancreatitis it has been proposed many hypotheses. Some of them were eventually dismissed, partially confirmed by other clinical and experimental research. Significant progress in understanding the process of fibrosis in the pancreas is associated with the identification, isolation and description of pancreatic stellate cells. They present in periacinar space and have long cytoplasmic processes covered the basis of acinus. This cells can be changed from stable fat-storing to miofibroblastic phenotype. Pancreatic stellate cells perform a wide range of functions, they have the ability to contraction, proliferation, they can synthesize extracellular matrix components and influence on the surrounding cellular environment. These cells can be regarded as a morphological basis for the development of pancreatic fibrosis. Currently, for the treatment of chronic pancreatitis, the main therapy is directed on depression of secretory activity of pancreas and inactivation biogenic amines in blood. Treatment of chronic pancreatitis should aim to influence the key mechanisms of pancreatic stellate cells activation and proliferation. Understanding the biology of this cells can open potential therapeutic targets for treatment and prevention of chronic pancreatitis and other diseases accompanied by pancreatic fibrosis.

Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 Peculiarities of treatment physical cultural and laserotherapy using for regulation integrative function in children with cerebral palsy Особливості застосування лікувальної фізкультури та лазеротерапії у хворих на дитячий церебральний параліч з позиції впливу на регуляцію вищих інтегративних функцій 13 16 UK V. P. Guray N. U. Grishunina T. V. Kornilova 2010 2 18 2010 3 21 The rehabilitation of children with cerebral palsy with using for treatment physical culture on basis of dynamics stereotypes structure impairment and lasers therapy was carried out. Influence a complex treatment to psycho-intellections functions, motors and vegetative providing of activity has been estimated. Somatical, neurological condition and vegetative providing of activity 30 children 7-11 years old has been studied. Depended on form using method 20 patients was divided into two groups. First group of complex treatment correction physical culture, treatments massage and laseroacupuncture was used. Second group of traditional methods of therapy and massage was used. The third group consisted of health children. The results achieved testly demonstrated rehabilitations complex promotion to development of compensatory mechanisms.

Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 Changes of pancreatic structure in conditions of an acute pancreatitis modelling Зміни структури підшлункової залози в умовах моделювання експериментального гострого панкреатиту 17 21 UK D. Yu. Zinenko T. V. Sukhina 2009 12 21 2010 1 14 Used various variants of 2 models: 1) model with intraperitoneal introduction L-arginin in dozes 3; 4 and 5 g/kg; 2) model with introduction 50 mkl 1 %-s', 2,5 %-s' and 5 %-s' solutions of sodium taurocholat in a channel of a pancreas. Hystologic research and definition of an amylases level in the blood carried out in 1, 4, 8, 12, 24, 48 and 72 hours after an injection. The lead researches on models with intraperitoneal introduction L-arginin and intrachannel introduction of sodium taurocholat have revealed a similar orientation in development of a sharp pancreatitis on dynamics of an amylases level in the blood and on pathomorphological changes of the parenchyma, stroma and microcirculation in the body. For model with introduction of sodium taurocholat in a channel of a pancreas are more characteristic gemorragycal displays alongside with inflammatory and necrotic changes which have an essential gradient from the head to a tail of the gland. It is observed regenerative dynamics of amylase’s concentration in the blood condition, of microvessels and the general tissue structure of a pancreas of comparison with L-аrginin after application of sodium taurocholat.

Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 Spatial reconstruction of facial skin capillaries Просторова реконструкція гемокапілярів шкіри обличчя 22 25 UK O. I. Makarchuk 2009 12 17 2010 2 15 To define structural and functional changes of skin capillaries in women of different age groups in this work intraoperational biopsy material of skin of 205 women at the age from 19 to 75 years, that was taken during standard surgery instrumentations for different defects of face and neck skin correction, was investigated. Skin material of cheek face region, temple region of head and anterior neck region was morphologically processed. To define parameters of dermal capillars and spatial reconstruction of intrapapillary capillary loops, serial sections was investigated with the help of morphometry. It was determined, that microcirculation age changes include structural disorders of intrapapillary capillary loops. Essential structural and functional changes observed in skin of cheek region in women of 33-40 years and in temple region of head and anterior neck region in women of 41-50 years. It is typical at the patients with nicotinic dependence, ischemic heart disease, hypertonic disease, a diabetes, and also adiposity of a different degree essential infringement of microvessels bed structure of a skin that gives the basis for allocation of the given contingent of patients as group high intraoperative and postoperative risk at carrying out of operative interventions for correction of face skin involutive changes.

Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 An effect of wrapping peripheral nerve anastomosis with pedicled muscle flap on nerve regeneration in experiment Морфологическая оценка влияния нейрорафии с миотизацией анастомоза на регенерацию периферического нерва в эксперименте 26 32 RU L. Yu. Naumenko A. N. Domanskiy V. I. Shponka 2019 2 11 2010 3 1 Despite intrinsic capacity of peripheral nerves to regenerate, functional outcomes of peripheral nerves injury remain poor. Nerve ischemia, intra-/perineurial fibrosis and neuroma formation contribute a lot to that. Several authors demonstrated beneficial effects of increased vascularization at the site of injury on peripheral nerves regeneration. The use of highly vascularized autologous tissues (greater omentum) as a source of peripheral nerves neovascularization shows promising results. We proposed a surgical technique in which injured peripheral nerves anastomosis was wrapped in a pedicled muscular flap and performed morphological assessment of the efficacy of such technique with the aid of immunohistochemistry. 14 rats (which underwent sciatic nerve transection) were operated according to proposed technique. Another 14 rats, in which only end-to-end nerve anastomosis (without muscular wrapping) was performed served as controls. Morphological changes were evaluated at 3 weeks and 3 months periods. Higher blood vessel and axon counts were observed in experimental groups at both checkpoints. There was also an increase in Schwann cells and macrophages counts, and less collagen content in peripheral nerves of experimental groups. Axons in neuromas of experimental groups showed a higher degree of arrangement. We conclude that proposed surgical technique provides better vascularisation of injured peripheral nerves, which is beneficial for nerve regeneration.

Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 The reproductive system function at women athletes are training by lifting and taekwondo Репродуктивна функція спортсменок, які займаються важкою атлетикою та тхеквондо 33 39 UK O. B. Nekhanevich 2010 3 4 2010 3 22 The purpose of investigation is studying the character and leading mechanisms of reproductive system pathology at women athletes are training by lifting and taekwondo. The object of research was the medical control at women are training in lifting and taekwondo. The results of the research have shown that the physical and psychoemotional overexertion with restriction of eating lead to adrenocorticotropic horomon considerable quantity emission which stimulates suprarenal glands and cause the development of hyperanrogenemy and ovaries functions depression. The athletes have more frequent menstrual cycle disturbances such as opsomenorrhea, oligomenorrhea and algodysmenorrhea. Typically there are changes of indices of physical development, appearance the symptoms of psychological masculinithation, hirsutism and secondary ovaries disturbance.

Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 Іmmunohistochemical characteristics of cellular populations and factors of sclerosis development on the late stages of localized scleroderma. Иммуногистохимическая характеристика клеточных популяций и факторов развития склероза на поздних стадиях ограниченной склеродермии 40 46 RU K. V. Romanenko 2010 3 4 2010 3 22 The purpose of the current study was to reveal immunohistochemical features of derma and epidermis in the late stage of localized scleroderma using markers: CD3, CD8, CD20, CD79α, CD68, CD1α, CD34, CD105, αSMA, vimentin, eNOS, Ki67, collagen IV, bcl2, caspase 3. It was determined that as far as inflammatory processes weakens there are the followings changes in mononuclear infiltrates: partial substitution of T-limphocytes on B, predominance of mature B-lymphocyles, increase in T-supressors amount. The eNOS activity decreases constantly. The CD105 marker prevails in endothelium. CD34+ dendrocytes in papillary and superficial areas of the dermal reticular layer reduce in number constantly that results in forming of gradient. CD1α+, vimentin+ and αSMA+ of dendritic cells are maximal in number on the stage of edema and induration and break down on the stage of sclerosis. The change of cellular composition was more substantial round the appendages of skin. Till the sclerosis stage Ki67+ cells amount decreases substantially, as well as caspase 3+ and bcl2+ cells, both among immune and dendritic cells.

Ukrainian Scientific Society of Anatomists, Histologists, Embryologists Morfologiâ 1997-9665 4 1 2010 07 14 The immunohistochemical estimation of local immune reaction in chronic granulating periodontitis Иммуногистохимическая оценка местного иммунитета при лечении хронического гранулирующего периодонтита 47 53 RU Yu. V. Sidash 2010 2 4 2010 3 12 The purpose of the current study was to establish quantitative dynamics of immune cells and to study proliferative activity in pathologic foci before and after treatment of chronic granulating periodontitis. Before treatment the number of CD3+, CD4+, CD20+, CD138+, CD68+ cells had statistically reliable distinctions in comparison with the control. CD4+/CD8+ lymphocytes ratio was 0.87. The share of plasma cells among all B-lymphocytes was 37.8%. After traditional treatment of granulating periodontitis only quantity of CD68+ cells was not statistically different from the control. CD4+/CD8+ lymphocytes ratio increased on 0.02, the share of plasma cells - to 43.8 %. After original complex treatment all studied parameters, excepting Ki67+ cells, reached the level, statistically not different from the control. CD4+/CD8+ lymphocytes ratio increased on 0.05, and CD138+/CD20+ lymphocytes ratio increased to 0.53. The most prominent changes within granular tissue after treatment occurred in distribution of B-lymphocytes.